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Primary structure of a major sialyl‐saccharide alditol from human amniotic mucins expressing the tumor‐associated sialyl‐X antigenic determinant
Author(s) -
Hanisch Franz-Georg,
Egge Heinz,
Peter-Katalinic Jasna,
Uhlenbruck Gerhard
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80507-5
Subject(s) - exoglycosidase , chemistry , fast atom bombardment , mucin , glycolipid , antigen , amniotic fluid , methylation , mass spectrometry , glycan , biochemistry , microbiology and biotechnology , chromatography , glycoprotein , biology , dna , immunology , pregnancy , fetus , genetics
A major sialyl‐saccharide alditol isolated from mucins of human amniotic fluid was purified by high‐performance liquid chromatography followed by high‐performance thin‐layer chromatography of the methylated derivative and subjected to structural analyses which comprised fast atom bombardment and electron impact‐mass spectrometry, methylation analysis, exoglycosidase digestion and CrO 3 oxidation. The structure of a disialylated pentasaccharide characterized by a sialyl‐X antigenic determinant was established: