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Expression of the cloned subunits of Escherichia coli transhydrogenase from separate replicons
Author(s) -
Clarke David M.,
Bragg Philip D.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80503-8
Subject(s) - escherichia coli , plasmid , biochemistry , atpase , enzyme , biology , chemistry , mutant , microbiology and biotechnology , gene
The pntA and pntB genes of Escherichia coli , encoding the α‐ and β‐subunits of the pyridine nucleotide transhydrogenase, were cloned individually in two different compatible plasmids into Escherichia coli mutants lacking transhydrogenase activity. Energy‐linked and non‐energy‐linked transhydrogenase activities were produced only in cells carrying both plasmids thus showing that the products of both genes are required for the formation of an active enzyme. ATP‐energized transhydrogenase activity was not increased in cells containing amplified levels of the transhydrogenase when the cell membrane ATPase was also amplified. It is suggested that the excess transhydrogenase is effectively uncoupled from the ATPase by compartmentalization in the cell.