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The occurrence and distribution of α‐hydroxy‐acid dehydrogenase in some members of the order Kinetoplastida
Author(s) -
Taylor Mark B.,
Gutteridge Winston E.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80487-2
Subject(s) - trypanosoma cruzi , gossypol , biology , biochemistry , dehydrogenase , lactate dehydrogenase , kinetoplastida , enzyme , cytosol , organelle , microbiology and biotechnology , parasite hosting , world wide web , computer science
LDH‐X is the isoenzyme of lactate dehydrogenase found in mammalian spermatozoa, occurring in cytosolic and mitochondrial locations. Gossypol strongly inhibits it, and the spermicidal action of this compound is attributed to the disruption of a reducing shuttle. The flagellated protozoan, Trypanosoma cruzi , contains an enzyme activity similar to LDH‐X, called α‐hydroxy‐acid dehydrogenase, which is here shown to possess cytosolic and glycosomal components. The glycosome is a microbody‐like organelle containing the early glycolytic enzymes. We postulate that the inhibition of replication of T. cruzi by gossypol derives from interference with glycosomal reducing shuttles. T. lewisi resembles T. cruzi in this respect.