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Pyruvate utilization by synaptosomes is independent of calcium
Author(s) -
Kauppinen Risto A.,
Nicholls David G.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80484-7
Subject(s) - veratridine , synaptosome , chemistry , calcium , biochemistry , pyruvate decarboxylation , biophysics , pyruvate carboxylase , biology , membrane , sodium , enzyme , sodium channel , organic chemistry
The significance of Ca 2+ is assessed for the activation of pyruvate oxidation by intact nerve terminals (synaptosomes). Titration of glucose‐depleted synaptosomes with pyruvate in the presence of either veratridine or uncoupler stimulates respiration in a Ca 2+ ‐independent manner. Additionally, the ability of exogenous pyruvate to support the mitochondrial membrane potential in situ is independent of Ca 2+ . It is concluded that Ca 2+ does not regulate pyruvate oxidation in intact synaptosomes.