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Acetylcholine‐ and inositol 1,4,5‐trisphosphate‐induced calcium mobilization in Xenopus laevis oocytes
Author(s) -
Nadler Etta,
Gillo Boaz,
Lass Yoram,
Oron Yoram
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80481-1
Subject(s) - inositol , acetylcholine , xenopus , second messenger system , calcium , inositol trisphosphate , endocrinology , calcium in biology , medicine , muscarinic acetylcholine receptor , intracellular , inositol trisphosphate receptor , chemistry , inositol phosphate , biology , biophysics , microbiology and biotechnology , biochemistry , receptor , gene
Acetylcholine induces a complex electrical membrane response in Xenopus laevis oocytes. This response is mimicked, and probably mediated by injected inositol 1,4,5‐trisphosphate. Oocytes prelabelled with 45 Ca released calcium in two phases, the second, slow phase exhibiting first order kinetics of release. Brief exposure of prelabelled oocytes to acetylcholine resulted in a significant increase in the rate of calcium release that returned to control values 2‐3 min following the removal of the neurotransmitter. Intracellular injection of inositol 1,4,5‐trisphosphate resulted in increased rate of calcium release similar to, but longer than that caused by acetylcholine. Experiments conducted on single oocytes permitted the investigation of the relationship between acetylcholine‐induced and inositol 1,4,5‐trisphosphate‐induced calcium mobilization and the resulting electrical membrane response. Our data reinforce our previous suggestion that inositol 1,4,5‐trisphosphate is the intracellular second messenger of the muscarinic membrane electrical response in Xenopus oocytes.