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Preclinical increase in activity of muscle microsomal trypsin‐like protease in murine muscular dystrophy, C57BL/10‐mdx
Author(s) -
Sawada Hitoshi,
Tsuji Shigekatsu,
Kusumoto Shigeo,
Doi Yoshio,
Matsushita Hiroshi
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80478-1
Subject(s) - muscular dystrophy , mdx mouse , trypsin , protease , microsome , skeletal muscle , enzyme , duchenne muscular dystrophy , biochemistry , medicine , chemistry , endocrinology , biology , dystrophin
Preclinical alterations of protease activities in skeletal muscles from 10‐day‐old dystrophic mouse, C57BL/10‐mdx, were examined by using 10 fluorogenic peptide substrates. Among the activities tested, only Boc‐Val‐Pro‐Arg‐MCA‐hydrolyzing enzyme of the muscle microsomes showed an about 6‐fold higher level of activity in mdx mouse. The increase in activity was not observed in tissues other than skeletal muscle. The enzyme had a pH optimum between 8.5 and 11.0, and was inhibited with DFP and variety of trypsin inhibitors. The enzymatic activity transiently increased at 1‐2 weeks of age, the preclinical or very early stage of the disease. These results imply that the increased level of a trypsin‐like protease possibly present in muscle microsomes may be closely related to the manifestation of muscular dystrophy.