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Stereospecific antibodies to propranolol
Author(s) -
Wang Lynn,
Chorev Michael,
Feingers Judith,
Levitzki Alexander,
Inbar Michael
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80474-4
Subject(s) - stereospecificity , propranolol , antibody , chemistry , immunology , biochemistry , biology , medicine , catalysis
The β‐adrenergic antagonist propranolol was activated through its side chain, coupled to bovine serum albumin, and injected into BALB/c mice. After fusion of the splenocytes from these immunized mice with the NS‐1 myeloma cell line, two hybridomas, producing monoclonal anti‐propranolol antibodies, were isolated. Clone P‐49 was monospecific for propranolol, with a significant preference for the 1‐stereoisomer, as compared to the d form. On the other hand, clone P‐28 cross‐reacted with alprenolol as well as some other β‐antagonists. Both classes of antibodies competed with A431 epidermoid carcinoma β 2 ‐adrenoceptors for the binding of [ 3 H]propranolol. When ascites cells from clone P‐28 were fixed with glutaraldehyde, the anti‐propranolol monoclonal antibody became cell bound. These cell‐bound P‐28 antibodies bind propranolol and other β‐adrenergic ligands with a similar ranking order to the soluble monoclonal antibody. The cell‐bound antibody displayed a 5‐fold higher affinity towards 1‐propranolol than the soluble monoclonal antibody. The practical implications of these findings are discussed.