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Effects of gibberellic acid and abscisic acid on levels of translatable mRNA (1→3,1→4)‐β‐D‐glucanase in barley aleurone
Author(s) -
Mundy John,
Fincher Geoffrey B.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80434-3
Subject(s) - abscisic acid , gibberellic acid , aleurone , messenger rna , polyadenylation , biochemistry , biology , enzyme , chemistry , botany , gene , germination
Polyadenylated mRNA has been isolated from barley aleurone layers incubated in the presence and absence of exogenous gibberellic acid and abscisic acid. Immunoprecipitation of in vitro translation products with specific antibodies shows that low levels of translatable mRNA encoding (1 →3,1 →4)‐β‐D‐glucanase 4‐glucanohydrolase (EC 3.2.1.73) can be detected in the absence of the phytohormones. Gibberellic acid causes a 10‐fold increase in levels of translatable mRNA for the enzyme, while abscisic acid treatment suppresses the relative abundance of translatable (l →3,1 →4)‐β‐D‐glucanase mRNA.