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Molecular cloning of unintegrated closed circular DNA of porcine retrovirus
Author(s) -
Suzuka Iwao,
Shimizu Nobuaki,
Sekiguchi Kiichi,
Hoshino Hiroo,
Kodama Michi,
Shimotohno Kunitada
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80432-x
Subject(s) - retrovirus , insert (composites) , restriction enzyme , microbiology and biotechnology , southern blot , dna , biology , cloning (programming) , molecular cloning , virology , vector (molecular biology) , complementary dna , virus , gene , genetics , recombinant dna , mechanical engineering , computer science , engineering , programming language
Viral DNA of unintegrated closed circular form was isolated from a swine kidney cell line (SKL) which was infected with a porcine retrovirus Tsukuba‐1 (PRetV) produced from a swine malignant lymphoma‐derived cell line, Shimozuma‐1 and cloned using a λ phage vector; Charon 21 A. One of ten independent clones contained the 8.3 kb DNA fragment as an insert, which was thought to be a full length of viral DNA molecule carrying a long terminal repeat (LTR) sequence. We have analyzed this insert by mapping the recognition sites of some restriction endonucleases by Southern blot hybridization with appropriate probes.