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Muscarinic stimulation of inositol phosphate accumulation and acid secretion in gastric fundic mucosal cells
Author(s) -
Baudière Bruno,
Guillon Gilles,
Bali Jean-Pierre,
Jard Serge
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80429-x
Subject(s) - carbachol , muscarinic acetylcholine receptor , chemistry , endocrinology , medicine , stimulation , inositol phosphate , inositol , muscarinic agonist , basal (medicine) , atropine , intracellular , secretion , agonist , gastric glands , gastric acid , incubation , receptor , gastric mucosa , biology , biochemistry , stomach , insulin
The muscarinic agonist, carbachol (CCh), was shown to stimulate the production of inositol phosphates (IP) in isolated cells from rabbit fundic mucosa. This stimulatory effect was time‐ and dose‐dependent: EC 50 values for IP 1 , IP 2 and IP 3 accumulation were not statistically different. The mean value was 30 ± 8 μM ( n = 6). The corresponding maximal stimulation (% of basal value) observed after 20 min incubation in the presence of 100 μM CCh was 160 ± 15%. CCh‐induced IP accumulation was abolished by atropine ( K i = 0.32 ± 0.18 nM ( n =3)). The CCh concentrations leading to half‐maximal inhibition of N ‐[ 3 H]methylscopolamine binding and half‐maximal IP accumulation were similar. The half‐maximal value for CCh‐induced aminopyrine accumulation was 8‐times lower. These results indicate that IP 3 ‐mediated mobilization of intracellular Ca 2+ might be involved in CCh‐induced acid secretion by parietal cells.