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Time‐resolved measurements of fluorescence from reaction centres of Rhodopseudomonas viridis and the effect of menaquinone reduction
Author(s) -
Hörber J.K.H.,
Göbel W.,
Ogrodnik A.,
Michel-Beyerle M.E.,
Cogdell R.J.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80418-5
Subject(s) - bacteriochlorophyll , fluorescence , rhodopseudomonas , kinetics , photochemistry , chemistry , photosynthetic reaction centre , electron acceptor , rhodopseudomonas palustris , molecule , rhodospirillaceae , electron transfer , photosynthesis , pigment , biology , organic chemistry , bacteria , biochemistry , physics , optics , quantum mechanics , genetics
The kinetics of the fluorescence emitted by the ‘special pair’ of bacteriochlorophyll b molecules in reaction centres from Rhodopseudomonas viridis was recorded in the near infrared, with a time resolution of 1 ns. In nonreduced reaction centres two decay components were resolved with lifetimes of <0.5 and 2.5 ns. Upon reduction of the menaquinone electron acceptor three decay components were detected with lifetimes of < 0.5, 2.5 and 15ns.