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Transient and intramembrane trapping of pumped protons in thylakoids
Author(s) -
Polle Andrea,
Junge Wolfgang
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80417-3
Subject(s) - thylakoid , photosystem ii , gramicidin , trapping , chemistry , proton , photochemistry , dcmu , proton transport , photosystem i , oxidizing agent , biophysics , chloroplast , biochemistry , membrane , photosynthesis , biology , physics , ecology , organic chemistry , quantum mechanics , gene
We measured flash‐induced rapid proton release into the lumen of thylakoids. We found that the proton‐trapping domains which were inducible by low concentrations of gramicidin and at alkaline pH [e.g. (1983) Biochim.Biophys.Acta 723, 294‐307] were each not restricted to a particular photosystem II complex. Instead, they were delocalized. Oxidizing conditions increased the buffering capacity of the domains from approx. 5 H + per photosystem II more than 2‐fold by shifting the p K towards acidity.