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Enhancement of fibroblast growth factor‐induced diacylglycerol formation and protein kinase C activation by colon tumor‐promoting bile acid in Swiss 3T3 cells
Author(s) -
Takeyama Yoshifumi,
Tanimoto Tetsuji,
Hoshijima Masahiko,
Kaibuchi Kozo,
Ohyanagi Harumasa,
Saitoh Yoichi,
Takai Yoshimi
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80353-2
Subject(s) - diacylglycerol kinase , protein kinase c , protein kinase a , chemistry , fibroblast growth factor , second messenger system , phorbol , biochemistry , kinase , biology , microbiology and biotechnology , signal transduction , receptor
A small amount (50–200 μM) of deoxycholate (DOC), a colon tumor‐promoting bile acid, did not show a direct effect on protein kinase C activity in a cell‐free system, but enhanced fibroblast growth factor (FGF)‐induced diacylglycerol formation and protein kinase C activation in Swiss 3T3 cells. DOC potentiated both reactions induced by submaximal doses of FGF but showed little effect on the maximal levels of the reactions. DOC alone was inactive in eliciting both reactions in the absence of FGF. DOC did not affect the binding of FGF to the cells. Since it has been described that diacylglycerol serves as a messenger for the activation of protein kinase C in the action of FGF in Swiss 3T3 cells [(1985) FEBS Lett. 191, 205‐210], these results suggest that a small amount of DOC increases the sensitivity to FGF of diacylglycerol formation and thereby potentiates protein kinase C activation in this cell line. This action of DOC was in marked contrast to that of 12‐ O ‐tetradecanoylphorbol‐13‐acetate, a potent tumor‐promoting phorbol ester, which directly activated protein kinase C in cell‐free and intact cell systems.