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Neomycin: a specific drug to study the inositol‐phospholipid signalling system?
Author(s) -
Prentki Marc,
Deeney Jude T.,
Matschinsky Franz M.,
Joseph Suresh K.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80343-x
Subject(s) - neomycin , inositol , phospholipid , intracellular , biochemistry , chemistry , phosphatidylinositol , antibiotics , signal transduction , membrane , receptor
Neomycin, an antibiotic previously thought to interact specifically with inositol‐containing phospholipids, was found to inhibit IP 3 ‐mediated Ca 2+ release from the intracellular stores of permeabilized insulinoma and liver cells. This inhibition could be relieved by increasing the IP 3 concentration. Radiolabelled IP 3 was found to bind tightly to columns prepared from neomycin covalently attached to glass beads. ATP was also bound by these colums. It is concluded that neomycin acts in biological systems as a weak anion exchanger and is therefore unsuitable for use as a specific tool to study the role of inositol phospholipids in intracellular signalling.