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Selective detection of rapid motions in spectrin by NMR
Author(s) -
Fung L.W.-M.,
Lu H.-Z.,
Hjelm R.P.,
Johnson M.E.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80333-7
Subject(s) - spectrin , chemistry , molecule , resonance (particle physics) , envelope (radar) , spectral line , proton , pulse (music) , solvent , nuclear magnetic resonance , crystallography , biochemistry , atomic physics , physics , cytoskeleton , organic chemistry , telecommunications , radar , astronomy , quantum mechanics , detector , computer science , optics , cell
Human erythrocyte spectrin molecules exhibit relatively sharp (30–50 Hz) proton NMR signals in the aliphatic region. A standard solvent presaturation pulse sequence that also partially suppresses the broad envelope from protons with rigid structures in spectrin and selectively enhances the sharp resonances has been used to characterize the behavior of these resonances. The overall resonance pattern strongly resembles that of the denatured spectrin. The observed spectra are also quite similar to the line‐broadened spectrum from a mixture of amino acids that corresponds to the composition of the spectrin molecule. These data indicate the existence of regions exhibiting rapid internal motions within the intact spectrin molecule, and suggest that the amino acid composition of the residues giving rise to the sharp resonances is quite similar to that of the full spectrin molecule.