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Localization of elongation factor Tu on the ribosome
Author(s) -
Girshovich A.S.,
Bochkareva E.S.,
Vasiliev V.D.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80325-8
Subject(s) - immunoelectron microscopy , ribosome , ef tu , protein subunit , ribosomal rna , elongation factor , chemistry , microbiology and biotechnology , biology , biophysics , biochemistry , genetics , rna , gene , antibody
The EF‐Tu‐binding center of the E. coli ribosome has been localized by immunoelectron microscopy after cross‐linking of the specific EF‐Tu · 70 S ribosomal complex with dimethylsuberimidate. EF‐Tu has been found to be in contact with the 50 S subunit in the region of the L7/L12 stalk and with the 30 S subunit in the upper part of its body on the side opposite the top of the ledge (the platform). The EF‐Tu position on a model of the 70 S ribosome is presented.