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Identification of a 27‐kDa enkephalin‐containing protein associated with bovine adrenal medullary chromaffin granule membranes by immunoblotting
Author(s) -
Birch Nigel P.,
Davies Anne D.,
Christie David L.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80321-0
Subject(s) - chromaffin cell , membrane , molecular mass , adrenal medulla , lysis , chemistry , biochemistry , granule (geology) , enkephalin , membrane protein , neuropeptide , biology , endocrinology , enzyme , catecholamine , paleontology , receptor , opioid
An antiserum which recognizes high molecular mass enkephalin‐containing proteins was used to compare proenkephalin intermediates in both the soluble and membrane components of bovine adrenal chromaffin granules by immunoblotting. While a range of molecular mass forms were identified in the soluble lysate the major form in the membranes corresponded to a 27‐kDa enkephalin‐containing protein. Enzymic digestion of bands of 27‐kDa material and quantitation of the enkephalin released showed that 22% of this material was membrane‐associated. High concentrations of chaotropic agents were required to extract this material from the membranes. Association of hormone and neuropeptide precursors with membrane components may by important for targeting of precursors to secretory granules or correct processing.