Premium
Formation of metabolites of [Arg 8 ]vasopressin (AVP) by brain peptidases
Author(s) -
Wang Xin-Chang,
Burbach J.Peter H.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80319-2
Subject(s) - vasopressin , aminopeptidase , chemistry , endocrinology , medicine , metabolism , neuropeptide , biochemistry , membrane , biology , amino acid , leucine , receptor
[Cyt 6 ]AVP‐(3–9), an intermediate in the metabolism of AVP‐(1–9) in vitro, was used to investigate the mechanism of formation of centrally active AVP metabolites. Exposure of [Cyt 6 ]AVP‐(3–9) to rat brain membranes resulted in formation of [Cyt 6 ]AVP‐(4–9),‐(5–9), [pGlu 6 ,Cyt 6 ]AVP‐(4–9) and AVP‐(3–5), which were isolated and chemically identified. Products derived from cleavage of the C‐terminus of the substrate were absent. Time‐course experiments further indicated that the conversion process is predominantly mediby an aminopeptidase‐like mechanism. The conversion of [Cyt 6 ]AVP‐(3–9) by membranes from hippocampus, amygdala and septum was quantitatively and qualitatively similar. The results point to a major role of aminopeptidase activity in the metabolic conversion of AVP and the formation of centrally active AVP metabolites.