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Quantitative changes in the catalytic and regulatory subunits of nuclear cAMP‐dependent protein kinases type I and type II during isoproterenol‐induced growth of the rat parotid gland
Author(s) -
Schwoch Gerhild,
Freimann Astrid
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80315-5
Subject(s) - protein subunit , antiserum , kinase , enzyme , parotid gland , agonist , microbiology and biotechnology , chemistry , immunoassay , protein kinase a , medicine , endocrinology , biochemistry , biology , receptor , antibody , immunology , gene , pathology
To quantify the cAMP‐dependent protein kinases I and II in parotid gland nuclei independent of the enzyme activity, monospecific antisera against their subunits were applied in a sensitive enzyme immunoassay. About 3% of total catalytic subunit in the homogenate was found in the isolated nuclei. During β‐agonistinduced proliferation of the parotid gland the nuclear concentration of catalytic and regulatory subunits changed. Related to the number of nuclei, the catalytic subunit and the regulatory subunit RI increased about 3‐fold whereas the regulatory subunit RII remained unchanged.