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A chemiluminescent probe with a Cypridina luciferin analog, 2‐methyl‐6‐phenyl‐3,7‐dihydroimidazo[1,2‐a]pyrazin‐3‐one, specific and sensitive for O 2 − production in phagocytizing macrophages
Author(s) -
Sugioka Katsuaki,
Nakano Minoru,
Kurashige Satonori,
Akuzawa Yuki,
Goto Toshio
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80291-5
Subject(s) - chemiluminescence , luciferin , chemistry , photochemistry , stereochemistry , medicinal chemistry , luciferase , organic chemistry , biochemistry , transfection , gene
When a Cypridina luciferin analog (the title compound) was added to a macrophage suspension in Hank's balanced salt solution (control), the system emitted a weak, but detectable light, which was not altered in the presence of Superoxide dismutase. The same system, however, emitted a much stronger light, just after the addition of a trigger, opsonized zymosan. The luminescence was suppressed to the control level in the presence of superoxide dismutase, while it was only slightly influenced, if at all, by NaN 3 , a scavenger of singlet oxygen and an inhibitor of myeloperoxidase. Some other results obtained also indicate the participation of O 2 − in the luciferin analog‐dependent luminescence in macrophages during phagocytosis.