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Platelet‐activating factor mobilises intracellular calcium in vascular smooth muscle cells
Author(s) -
Doyle Valerie M.,
Creba Judith A.,
Rüegg Urs T.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80288-5
Subject(s) - inositol , intracellular , platelet activating factor , efflux , inositol phosphate , vascular smooth muscle , calcium , calcium in biology , chemistry , inositol trisphosphate , microbiology and biotechnology , platelet , endocrinology , medicine , biochemistry , smooth muscle , biology , receptor , organic chemistry
The effect of platelet‐activating factor (PAF) on polyphosphoinositide metabolism and 45 Ca 2+ efflux was examined in a vascular smooth muscle cell line (A 7 r 5 ). PAF stimulated a rapid but transient production of inositol trisphosphate and inositol bisphosphate which, in the presence of lithium, resulted in an accumulation of inositol monophosphate. In addition, PAF induced a rapid efflux of 45 Ca 2+ from preloaded cells, an effect which was concentration‐dependent. These data suggest that PAF mobilises intracellular Ca 2+ via the production of inositol trisphosphate.