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Transglutaminase activity and putrescine‐binding capacity in cloned cell lines with different metastatic potential
Author(s) -
Delcros J.G.,
Bard S.,
Roch A.M.,
Quash G.,
Poupon M.F.,
Korach S.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80272-1
Subject(s) - tissue transglutaminase , putrescine , cell culture , fibronectin , binding protein , clone (java method) , microbiology and biotechnology , cell adhesion , biology , recombinant dna , plasma protein binding , biochemistry , cell , chemistry , enzyme , dna , genetics , gene
An inverse correlation was found between cellular transglutaminase activity and metastatic potential of four cloned cell lines derived from a primary nickel‐induced rat rhabdomyosarcoma. Cellular transglutaminase activity as assessed with endogenous cellular protein or exogenous methylated casein was greatest in the clone F9‐ which is the least metastasizing. When the putrescine‐binding capacity of one cellular derived protein ‐ fibronectin ‐ was examined with exogenous transglutaminase, it was found that the fibronectin derived from the clone F9‐ showed the lowest binding capacity compared with those from the other clones. However, when the overall binding capacity of cellular proteins from each cell line was examined no differences could be detected. The results are discussed in the light of the well‐known role of fibronectin in cellular adhesion.