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Primary structure of the β‐subunit of Torpedo californica (Na + + K + )‐ATPase deduced from the cDNA sequence
Author(s) -
Noguchi Shunsuke,
Noda Masaharu,
Takahashi Hideo,
Kawakami Kiyoshi,
Ohta Toshiko,
Nagano Kei,
Hirose Tadaaki,
Inayama Seiichi,
Kawamura Masaru,
Numa Shosaku
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80270-8
Subject(s) - complementary dna , protein primary structure , microbiology and biotechnology , cdna library , protein subunit , nucleic acid sequence , peptide sequence , biology , torpedo , transmembrane protein , biochemistry , dna , gene , acetylcholine receptor , receptor
DNA complementary to the Torpedo californica electroplax mRNA coding for the β‐subunit of (Na + + K + )‐ATPase has been cloned by screening a cDNA library with an oligodeoxyribonucleotide probe. Nucleotide sequence analysis of the cloned cDNA has revealed that this polypeptide consists of 305 amino acid residues (including the initiating methionine). The transmembrane topology and the potential N ‐glycosylation sites of this polypeptide are discussed.