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Cytoplasmic pH and glycolysis in the Dictyostelium discoideum cell cycle
Author(s) -
Aerts R.J.,
Durston A.J.,
Moolenaar W.H.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80235-6
Subject(s) - dictyostelium discoideum , glycolysis , cytoplasm , microbiology and biotechnology , chemistry , biochemistry , biophysics , biology , metabolism , gene
Lactate production measurements during the cell cycle of synchronized populations of Dictyostelium discoideum cells reveal cyclic variations in glycolysis which correspond with pH i oscillations which were discovered by us previously [(1985) Cell, in press]. Aerobic lactate production varies about 6‐fold during the cell cycle and the lactate maxima correlate with (~ 0.25 pH unit) cyclic increases in pH. However, artificially altering pH i using weak acids or bases does not influence the rate of lactate production in asynchronous cell populations. This result suggests that the cyclic variations in pH i and those in glycolytic rate are not causally related events.