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cAMP mediated proteolysis of the catalytic subunit of cAMP‐dependent protein kinase
Author(s) -
Hemmings Brian A.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80226-5
Subject(s) - protein subunit , protein kinase a , proteolysis , forskolin , kinase , chemistry , biochemistry , microbiology and biotechnology , biology , enzyme , in vitro , gene
The cAMP‐dependent protein kinase from LLC‐PK 1 cells can be activated in vivo by calcitonin and vasopressin, or forskolin. Continuous treatment of cells with these agents results in a decrease of total cAMP‐PK activity. The loss of kinase activity was enhanced when either of these three agents was incubated in the presence of isobutylmethylxanthine. Results obtained using affinity purified antibodies to the catalytic subunit show that the loss of kinase was due to specific proteolysis of this subunit.