Premium
Complete characterization of the human IGF‐I nucleotide sequence isolated from a newly constructed adult liver cDNA library
Author(s) -
Le Bouc Y.,
Dreyer D.,
Jaeger F.,
Binoux M.,
Sondermeyer P.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80223-x
Subject(s) - complementary dna , signal peptide , peptide sequence , cdna library , polyadenylation , nucleic acid sequence , microbiology and biotechnology , coding region , biology , rapid amplification of cdna ends , messenger rna , biochemistry , dna , gene , molecular cloning
A full‐size cDNA sequence coding for insulin‐like growth factor I (IGF‐I) was isolated from a human liver library. For the construction of this bank, a new method was developed which anneals dG‐tailed cDNA with a synthetic adaptor 5'‐AATT‐3' followed by ligation into the EcoRI site of a lambda immunity‐insertion vector. Based on the sequence analysis of the complete IGF‐I messenger we concluded that the protein is synthesized as a precursor containing a signal peptide of 22 or 25 amino acid residues. In addition, the sequence extended in the 3'‐direction and showed the presence of multiple polyadenylation sites in the IGF‐I message.