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Antibody‐coated liposomes
Author(s) -
Senior Judith,
Waters J.A.,
Gregoriadis Gregory
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80213-7
Subject(s) - liposome , antibody , monoclonal antibody , phospholipid , chemistry , incubation , immunoglobulin g , antigen , microbiology and biotechnology , biochemistry , immunology , biology , membrane
Incubation of small unilamellar liposomes composed ofequimolar phospholipid and cholesterol with mouse IgG or mouse monoclonal IgG 1 for up to 24 h resulted in considerable (34‐89%) adsorption of the protein onto the liposomal surface. Immunoglobulin remained adsorbed after exposure to mouse plasma and, in the case of monoclonal (anti‐HBSAg) IgG 1 , was able to mediate association of the liposomes with the antigen. Extensive immunoglobulin adsorption suggests caution in interpreting co valent linkage values obtained upon prolonged incubation of chemically modified antibodies with liposomes. It may also be a preferred alternative in preparring targeted liposomes for intravenous use when chemically modified antibodies promote premature clearance of the antibody‐liposome complex from the circulation.