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Express analysis of protein amino acid sequences
Author(s) -
Shlyapnikov S.V.,
Bezborodova S.I.,
Kulikov V.A.,
Yakovlev G.I.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80208-3
Subject(s) - edman degradation , penicillium chrysogenum , amino acid , rnase p , chemistry , peptide sequence , protease , biochemistry , peptide , enzyme , chromatography , rna , gene
The complete amino acid sequence of Penicillium chrysogenum 152A guanyl‐specific RNase has been established using automated Edman degradation of two non‐fractionated peptide mixtures produced by tryptic and staphylococcal protease digests of the protein. The RNase contains 102 amino acid residues: His 2 , Arg 3 , Asp 7 , Asn 8 , Thr 5 , Ser 11 , Glu 4 , Gln 2 , Pro 4 , Gly 11 , Ala 13 , Cys 4 , Val 8 , Ile 3 , Leu 3 , Tyr 9 , Phe 5 ( M r 10 747).