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Characterization and quantitative topographical distribution of salmon calcitonin‐binding sites in rat kidney sections
Author(s) -
Bouizar Z.,
Rostène W.H.,
Moukhtar M.S.,
Milhaud G.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80206-x
Subject(s) - calcitonin , receptor , binding site , chemistry , kidney , medulla , affinities , endocrinology , medicine , binding affinities , renal cortex , renal medulla , distribution (mathematics) , biology , biochemistry , mathematics , mathematical analysis
Renal binding sites for labelled salmon calcitonin (sCT) were studied using cryostat sections and autoradiography. Increasing concentrations of unlabelled sCT inhibited 125 I‐sCT binding. 125 I‐sCT bound to a single site with a K d of 2 nM and a number of sites of 220 protein. Mammalian calcitonins had low affinities and peptides unrelated to CT were devoid of any significant affinity for 125 I‐sCT receptors. Auto‐radiograms disclosed a high concentration of 125 I‐sCT receptors mainly located in the outer medulla and heterogeneously in the renal cortex. The distribution of specific binding sites is in agreement with the current concepts of renal action of calcitonin.