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Protein kinase C in cytosol and cell membranes of concanavalin A‐stimulated rat thymocytes
Author(s) -
Averdunk R.,
Günther T.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80193-4
Subject(s) - concanavalin a , cytosol , protein kinase c , protein kinase a , biochemistry , membrane , microbiology and biotechnology , kinase , biology , chemistry , enzyme , in vitro
In concanavalin A‐treated thymus cells and phytohemagglutinin‐treated spleen cells, the distribution of protein kinase C is changed. Shortly after addition of plant lectins, the activity of protein kinase C increased in the cytosol and decreased in the particulate fraction. 2 h later, the activity of protein kinase C decreased in the cytosol and increased in the particulate fraction. Membrane binding of protein kinase C and tion of DNA synthesis showed the same dependency on concanavalin A concentration. A long‐term membrane binding of protein kinase C seems to be essential for lymphocyte stimulation.