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Facilitated transport of 3‐ O ‐methyl‐D‐glucose in human polymorphonuclear leukocytes
Author(s) -
Okuno Yasuhisa,
Plesner Liselotte,
Larsen Thomas R.,
Gliemann Jørgen
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80182-x
Subject(s) - intracellular , cytochalasin b , extracellular , glucose transporter , chemistry , hexose , biochemistry , biophysics , cytochalasin , biology , endocrinology , cell , enzyme , cytoskeleton , insulin
Transport of the nonmetabolizable hexose analogue 3‐ O ‐methyl‐D‐glucose (3OMG) was measured in human polymorphonuclear leukocytes at 37° pH 7.4. 30MG at very low concentration (0.05 mM) equilibrated with the intracellular water with a rate constant of about 0.08 s −1 . Transport of 3OMG in the presence of 20 μM cytochalasin B and transport of L‐glucose were insignificant. Countertransport of 14 C‐labelled 3OMG was demonstrated. Exchange of 3OMG between the extracellular and intracellular water showed saturation with a K m of about 4 mM. Thus, the transport of 3OMG is mediated almost exclusively by facilitated diffusion.