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Effect of 2,4‐dinitrofluorobenzene on the enzymatic properties of the b ‐ c 1 complex isolated from beef heart mitochondria
Author(s) -
Lorusso M.,
Marzo M.,
Gatti D.,
Papa S.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80181-8
Subject(s) - enzyme , protein subunit , chemistry , mitochondrion , chemical modification , biochemistry , labelling , stereochemistry , gene
A study is presented on the effect of 2,4‐dinitrofluorobenzene (DFNB) on the enzymatic properties of mitochondrial b ‐ c 1 complex. The chemical modification by DNFB strongly inhibits the reductase activity of the complex, this being accompanied by labelling by [ 3 H]DNFB of core protein I, the apoprotein of b cytochromes and the 12 kDa subunit. Chemical modification by DNFB appears to alter, in particular, the domain of heme b ‐562.