Premium
Do the higher oxidation states of the photosynthetic O 2 ‐evolving system contain bound H 2 O?
Author(s) -
Radmer Richard,
Ollinger Otto
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80178-8
Subject(s) - photosynthesis , chemistry , biochemistry
A modified mass spectrometer was used to determine whether the higher oxidation states of the photosynthetic O 2 ‐evolving system contain substrate water that is not freely exchangeable with the external medium. Our data indicated that the higher oxidation states contain no appreciable bound, non‐exchangeable H 2 O. This suggests that H 2 O oxidation takes place via a rapid, concerted, all‐or‐none mechanism rather than by a mechanism involving stable, partially oxidized, H 2 O‐derived intermediates. These findings set definite constraints on possible mechanisms of O 2 evolution.