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Improved purification and N‐terminal sequence of the 33‐kDa protein in spinach PS II
Author(s) -
Yamamoto Yasusi,
Hermodson Mark A.,
Krogmann David W.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80151-x
Subject(s) - spinach , chloroplast , biochemistry , peptide sequence , chemistry , n terminus , sequence (biology) , protein sequencing , chromatography , gene
Significant improvement was attained in the purification of a peripheral 33‐kDa protein from spinach chloroplasts by the use of butanol/water phase partitioning. Over 30% of the 33‐kDa protein in the chloroplasts was isolated in a short time. The amino acid sequence for the first 37 residues from the N‐terminus was determined by the automated sequencing of the purified protein.