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Rate of exchange of Cl − between the aqueous phase and its action site in the O 2 evolving reaction of photosystem II studied by rapid, ionic‐jump‐induced Cl − depletion
Author(s) -
Itoh Shigeru,
Iwaki Masaya
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80148-x
Subject(s) - photosystem ii , chemistry , spinach , ion , aqueous solution , ionic bonding , hill reaction , phase (matter) , ionic strength , inorganic chemistry , photosynthesis , chloroplast , biochemistry , organic chemistry , gene
Rates of release and binding of Cl − from/to its action site in the O 2 evolving reaction in photosystem II particles derived from spinach chloroplasts were estimated by measuring the suppression of O 2 evolution by salt addition (ionic‐jump) and its recovery by the readdition of Cl − . It was estimated that depletion and rebinding of Cl − were completed within a few seconds. These results suggest that the Cl − ‐action site is located in a space which is almost freely accessible to various ions in the outer medium, with no barrier to ion movements. These results can be explained by electrostatic attraction of Cl − to its action site, as was proposed in a study of anion effects on O 2 evolution [(1986) Plant Cell Physiol. 27, in press].