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Acetylcholine release from guinea pig caudate slices evoked by phorbol ester and calcium
Author(s) -
Tanaka Chikako,
Fujiwara Hiroshi,
Fujii Yoshio
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80146-6
Subject(s) - protein kinase c , acetylcholine , guinea pig , depolarization , chemistry , ionophore , phorbol , calcium , neurotransmitter , synaptosome , medicine , endocrinology , biochemistry , biology , signal transduction , receptor , organic chemistry
In guinea pig caudate slices preloaded with [ 3 H]choline, superfusion with medium containing Ca 2+ and the phorbol ester TPA (12‐ O ‐tetradecanoyl phorbol 13‐acetate) produced an increase in the Ca 2+ ‐dependent, depolarization‐evoked Ca 2+ influx. The effect of TPA was antagonized by polymyxin B and H‐7, both inhibitors of protein kinase C. The combination of TPA and calcium ionophore (A23187) provoked the release of ACh to a level equal to the maximal response induced by depolarizing stimuli. TPA alone had no significant effect on the release of ACh. These results suggest that protein kinase C probably plays a role in transmembrane signal transduction involved in the release of neurotransmitter from nerve terminals in the brain.