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Complete nucleotide and deduced amino acid sequences of rat L‐type pyruvate kinase
Author(s) -
Lone Yu-Chun,
Simon Marie-Pierre,
Kahn Axel,
Marie Joëlle
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80138-7
Subject(s) - untranslated region , complementary dna , primer extension , coding region , biology , microbiology and biotechnology , messenger rna , three prime untranslated region , nucleic acid sequence , primer (cosmetics) , peptide sequence , five prime untranslated region , amino acid , pyruvate kinase , biochemistry , gene , genetics , chemistry , enzyme , organic chemistry , glycolysis
Four overlapping cDNA clones for L‐type pyruvate kinase (PK‐L) were isolated from carbohydrate‐induced rat liver cDNA libraries. They contained all the coding sequence of the enzyme from the 7th codon and the entire 3'‐untranslated extension up to the poly(A) tail. The sequence of the first 7 codons and that of the 5'‐untranslated region were determined by primer extension. The analyzed PK‐L mRNA has 19 5'‐untranslated bases, 1629 coding bases and 1281 3'‐untranslated bases without the poly(A) tail; it corresponds to the heavier, 3.2 kb species of the L‐type mRNAs. The codons for the phosphorylatable site are, located at the 5'‐end of the messenger. The unusually long 3'‐untranslated extension contains a repetitive element complementary to the ‘brain‐specific’ identifier sequence described by Sutcliffe et al. [(1982) Proc. Natl. Acad. Sci. USA 79, 4942‐4946].