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Spectral properties of fluorescent flavoproteins of isolated rat liver mitochondria
Author(s) -
Kunz Wolfram S.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80137-5
Subject(s) - flavoprotein , flavin group , nad+ kinase , chemistry , mitochondrion , biochemistry , fluorescence , redox , photochemistry , enzyme , physics , inorganic chemistry , quantum mechanics
The different flavoproteins contributing to flavin fluorescence of isolated rat liver mitochondria have distinct excitation and emission spectra. The NAD‐linked flavin component was identified as α‐lipoamide dehydrogenase, while the non‐NAD‐linked component was found to be electron transfer flavoprotein. The differences in excitation and emission properties of the mitochondrial flavoproteins permit selective recording of their redox state changes in isolated mitochondria.