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Effects of microtubule‐associated proteins on network formation by neurofilament‐induced polymerization of tubulin
Author(s) -
Minami Yasufumi,
Sakai Hikoichi
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80132-6
Subject(s) - neurofilament , tubulin , microtubule , polymerization , cytoskeleton , chemistry , biophysics , microtubule associated protein , microbiology and biotechnology , biochemistry , biology , cell , immunology , organic chemistry , polymer , immunohistochemistry
It has been revealed that neurofilaments stimulate polymerization of tubulin and thereby cause gelation. Addition of a very small amount of MAPs to the reaction mixture of tubulin and neurofilaments resulted in promotion of gelation. This could not be ascribed to MAP‐induced cross‐linking between microtubules and neurofilaments because further increases in the MAP concentration (still substoichiometric amount) resulted in total suppression of gelation. It is concluded that MAPs promote microtubule assembly independently of neurofilaments, and lower the concentration of tubulin available for neurofilament‐induced polymerization, then preventing network formation.