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Flow cytometry and biochemical analysis of DNA degradation characteristic of two types of cell death
Author(s) -
Afanas'ev Vladimir N.,
Korol' Boris A.,
Mantsygin Yurii A.,
Nelipovich Piotr A.,
Pechatnikov Vladimir A.,
Umansky Samuil R.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80115-6
Subject(s) - flow cytometry , dna fragmentation , dna , apoptosis , programmed cell death , membrane permeability , microbiology and biotechnology , fragmentation (computing) , chemistry , cell , biology , biochemistry , membrane , ecology
By flow cytometry it has been shown that in rat thymocytes dying upon γ‐irradiation the reduction in DNA content per cell occurs well before the increase in outer membrane permeability. In contrast, in irradiated Burkitt's lymphoma cells the disturbance of plasma membrane permeability precedes the decrease of DNA content. Genome degradation in dying thymocytes of irradiated or hydrocortisone‐treated rats is accounted for by internucleosomal DNA fragmentation. Postirradiation DNA cleavage in the 3 investigated cell cultures is disorderly and is probably caused by the activation of hydrolases in dead cells.