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cDNA clones coding for the β‐subunit of human liver alcohol dehydrogenase have differently sized 3'‐non‐coding regions
Author(s) -
Heden Lars-Olof,
Höög Jan-Olov,
Larsson Kerstin,
Lake Mats,
Lagerholm Eva,
Holmgren Arne,
Vallee Bert L.,
Jörnvall Hans,
von Bahr-Lindström Hedvig
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80111-9
Subject(s) - coding region , complementary dna , nucleic acid sequence , polyadenylation , nucleotide , biology , cdna library , microbiology and biotechnology , genetics , gene , messenger rna
Three different size classes of cDNA clones coding for the β 1 ‐rsubunit of human alcohol dehydrogenase (ADH) were characterized from a human liver cDNA library. Clones were identified by hybridization with synthetic oligodeoxyribonucleotides. A total of 2530 nucleotides were determined, covering an ADH‐coding region of 1122 nucleotides, a preceding 72‐nucleotide segment and 3 types of 3'‐non‐coding region. The coding nucleotide sequence is in full agreement with the amino acid sequence of the β 1 subunit. Of 8 clones identified, 6 had a short, 213‐nucleotide 3'‐non‐coding region; 1 an intermediate, 590‐nucleotide 3'‐region; and 1 a long, 1330‐nucleotide 3'‐region. In addition, 2 unused polyadenylation signals were found. These results suggest that human liver β‐ADH mRNAs occur in several size classes, and that in addition to the consensus sequence AATAAA further signals are important for 3'‐end formation.