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Epidermolytic toxin from Staphylococcus aureus binds to filaggrins
Author(s) -
Smith Thomas P.,
Bailey Christopher J.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80107-7
Subject(s) - staphylococcus aureus , toxin , chemistry , microbiology and biotechnology , microbial toxins , staphylococcus , bacteria , biology , genetics
The affinity of epidermolytic toxin from Staphylococcus aureus for proteins from the target tissue has been tested by a Western blotting procedure. Particular proteins in a l M phosphate extract of epidermis reacted on nitrocellulose blots with a probe prepared by the conjugation of toxin with peroxidase. Protein extracted into 50 mM Tris‐HCl did not react. The probe detected profilaggrin, filaggrin and a smaller unidentified polypeptide. It is suggested that the interaction is relevant to the mode of action of the toxin.