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Nonenzymatic radiolabeling of protein by 32 P‐containing nucleotides
Author(s) -
Schmidt Martin C.,
Hanna Michelle M.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80106-5
Subject(s) - chemistry , nucleotide , photoaffinity labeling , nucleoside , cofactor , isotopic labeling , biochemistry , combinatorial chemistry , enzyme , organic chemistry , binding site , gene
We report a nonenzymatic reaction which results in the radiolabeling of proteins by 32 P‐containing nucleoside triphosphates. The labeling reaction does not require any cofactors, but is greatly enhanced by the presence of alcohols. Even under optimal conditions, less than 1% of the protein molecules undergo modification. This nonspecific labeling represents a serious artifact which may become significant in systems involving low levels of specific labeling, such as photoaffinity labeling. Since the reaction is not limited to specific proteins, this may, however, provide a simple and rapid procedure for the preparation of labeled proteins.