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M r determination of histones from their electrophoretic mobility in acidic urea gels in the absence of detergents
Author(s) -
Brandt Wolf F.,
Sewell B.T.,
von Holt Claus
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80099-0
Subject(s) - electrophoresis , urea , chemistry , polyacrylamide , chromatography , polyacrylamide gel electrophoresis , matrix (chemical analysis) , histone , biochemistry , polymer chemistry , gene , enzyme
The M r of histones can be determined from their electrophoretic mobility at pH 2.3, 8 M urea in a polyacrylamide gel by correcting for differences in their charge density and properties of the gel matrix. The applicability of this method to other proteins is considered.