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The resolution of five linker histone subtypes from chicken erythrocytes
Author(s) -
Harborne Nerina,
Allan James
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80098-9
Subject(s) - amberlite , elution , chemistry , chromatography , linker , histone , fractionation , sephadex , biochemistry , enzyme , dna , adsorption , computer science , organic chemistry , operating system
A method is described for the isolation of 4 highly purified H1 linker histone subfractions, and histone H5, from chicken erythrocytes. Fractionation is achieved under non‐denaturing conditions by elution from CMC‐25 Sephadex with an NaCl gradient. When applied to calf thymus H1, the order of subtype elution differs from that acheived on Amberlite IRC‐50 [(1966) J. Biol. Chem. 241, 5790‐5797; (1976) Biochemistry 15, 4233‐4242]. The two column types employed in series could provide a means for improving subtype purity.