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ANF stimulation of detergent‐dispersed particulate guanylate cyclase from bovine adrenal cortex
Author(s) -
Tremblay Johanne,
Gerzer Rupert,
Pang Stephen C.,
Cantin Marc,
Genest Jacques,
Hamet Pavel
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80086-2
Subject(s) - gucy1a3 , chemistry , stimulation , guanylate cyclase , adrenal cortex , gucy1b3 , guanylate cyclase 2c , gucy2d , endocrinology , medicine , biochemistry , biology , receptor
Particulate guanylate cyclase from bovine adrenal cortex can be stimulated by ANF. A 2‐fold stimulation of the enzyme was obtained with 100 nM ANF and a half‐maximal stimulation, with a 5 nM dose. The stimulation by ANF persisted for at least 30 min. Various detergents, such as Triton X‐100, Lubrol PX, cholate, CHAPS, digitonin and zwittergent, stimulated several‐fold the activity of particulate guanylate cyclase. However, only Triton X‐100 dispersed particulate guanylate cyclase without affecting its response to ANF. The dose‐response curve of ANF stimulation of the particulate and the Triton X‐100 dispersed enzyme was similar. The dispersion of a fully responsive guanylate cyclase to ANF will help us to uncover the type of interactions between guanylate cyclase and ANF. It will also be used as a first step for the purification of an ANF‐sensitive particulate guanylate cyclase.