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Sugar transport in Trypanosoma brucei : a suitable kinetic probe
Author(s) -
Game Stephen,
Holman Geoffrey,
Eisenthal Robert
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80063-1
Subject(s) - trypanosoma brucei , sugar , substrate (aquarium) , chemistry , biochemistry , carbohydrate metabolism , metabolism , glucose transporter , biology , microbiology and biotechnology , insulin , ecology , gene
A transport assay has been developed for use in the investigation of 1‐deoxy‐D‐glucose uptake in trypanosomes. 1‐Deoxy‐D‐glucose has high affinity for the trypanosome sugar transport system (net influx K m = 4.03 ± 0.42 mM; V = 0.052 ± 0.005 mM·s − . D‐Glucose oxidation is competitively inhibited by 1‐deoxy‐Dglucose. However, we show that 1‐deoxy‐D‐glucose is not a substrate for metabolism and that the competition occurs because of interaction at the transport system. D‐Glucose competitively inhibits 1‐deoxy‐D‐glucose influx.