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The roles of HPr and FPr in the utilization of fructose by Escherichia coli
Author(s) -
Kornberg Hans
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80042-4
Subject(s) - escherichia coli , fructose , enzyme , mutant , biochemistry , chemistry , transduction (biophysics) , enzyme assay , biology , microbiology and biotechnology , gene
A mutant impaired in FPr activity was isolated. The altered gene ( fpr ), which was located near min. 44 on the E. coli genome, was transferred by phage‐mediated transduction to appropriate recipients that lack HPr ( ptsH ), or Enzyme II man ( ptsM ), or neither. The rates of growth on fructose of such transductants indicate that phosphate from PEP is transferred predominantly via FPr to fructose that enters the cells by Enzyme II fru , but that HPr can play a role in transferring phosphate to fructose taken up via Enzyme II man