Premium
The excimer fluorescence of N ‐(1‐pyrenyl)iodoacetamide labeled to myosin and its subfragment 1
Author(s) -
Ikkai Takamitsu,
Mihashi Kohsin
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80035-7
Subject(s) - iodoacetamide , excimer , myosin , fluorescence , chemistry , biophysics , atpase , photochemistry , biochemistry , enzyme , biology , cysteine , physics , quantum mechanics
Myosin and its subfragment 1 were labeled with the fluorescent probe N ‐(1‐pyrenyl)iodoacetamide. Both of the labeled complexes exhibited the excimer band at 480 nm (pH 8.0, 25 °C). SH 1 and SH 2 are labeled with this probe as judged by Ca 2+ ‐ATPase of the labeled complex. Excimers arise both from the interaction of PIAAs in the two different heads within a single myosin molecule and also from the interaction of PIAAs in the same head. ATP affects these excimers depending on the concentration of Ca 2+ .