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Meta‐vinculin distribution in adult human tissues and cultured cells
Author(s) -
Glukhova M.A.,
Kabakov A.E.,
Belkin A.M.,
Frid M.G.,
Ornatsky O.I.,
Zhidkova N.I.,
Koteliansky V.E.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80027-8
Subject(s) - vinculin , vascular smooth muscle , myocyte , foreskin , microbiology and biotechnology , in vitro , aorta , cell culture , biology , medicine , endocrinology , focal adhesion , smooth muscle , biochemistry , signal transduction , genetics
Meta‐vinculin distribution in adult human tissue was studied by immunoblotting technique. Meta‐vinculin was found in smooth (aorta wall and myometrium) and cardiac muscle, rather than in skeletal muscle, liver, kidney and cultured cells ‐ macrophages, foreskin fibroblasts, peripheral blood lymphocytes and vascular endothelial cells. In the primary culture of smooth muscle cells from human aorta the meta‐vinculin/vinculin ratio was reduced, and on the onset of cell division meta‐vinculin could hardly be detected. Subcultured smooth muscle cells from human aorta did not contain meta‐vinculin. The data show that the presence of meta‐vinculin is characteristic of'contractile' smooth muscle cells rather than of proliferating in vitro.